AY 9944

Biochem/physiol Actions

Primary Target7-dehydrocholesterol reductase (δ7-sterol reductase)

Reversible: no

Product does not compete with ATP.

Target IC₅₀: 13 nM in blocking 7-dehydrocholesterol reductase (δ7-sterol reductase) activity

Cell permeable: yes

General description

A cell-permeable amphiphilic diamine that blocks cholesterol biosynthesis and its esterification. Specifically blocks 7-dehydrocholesterol reductase (δ⁷-sterol reductase) activity with IC₅₀= 13 nM. Important tool in Sonic Hedgehog signaling and teratogenicity studies. Also reported to induce a rapid and irreversible reduction in Acidic-Sphingomyelinase activity in fibroblasts.

A cell-permeable, amphiphilic diamine that acts as a potent inhibitor of cholesterol biosynthesis and esterification and exhibits teratogenic properties. Specifically blocks 7-dehydro-cholesterol reductase (δ⁷-sterol reductase; DHC) activity with IC₅₀= 13 nM. Reported to disrupt Sonic Hedgehog (Shh) signaling during embryogenesis in rats. Also reported to induce a rapid and irreversible reduction in acidic-sphingomyelinase activity in fibroblasts.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Gofflot, F., et al. 2001. Dev. Dyn.220, 99.Cooper, M.K., et al. 1998. Science280, 1603.Incardona, J.P., et al. 1998. Development125, 3553.Moebius, F.F., et al. 1998. Proc. Natl. Acad. Sci. USA95, 1899.Yoshida, Y. 1985. J. Biochem.98, 1669.Kraml, M., et al. 1964. Biochem. Biophys. Res. Commun.15, 455.

Packaging

5 mg in Glass bottle

Packaged under inert gas

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Harmful & Carcinogenic / Teratogenic (E)